Journal: bioRxiv

Article Title: Optimal Cannabinoid-Terpene Combination Ratios Suppress Mutagenicity of Gastric Reflux in Normal and Metaplastic Esophageal Cells

doi: 10.1101/2025.09.23.678062

Figure Lengend Snippet: (A)A representative immunofluorescence analysis of CB1 (red) and EPCAM (green) expression in the normal human esophageal mucosa (Magnification 63x). The inset highlights the CB1 expression in the basal epithelial layer. (B)A representative immunofluorescence analysis depicting CB1 and EPCAM co-expression in the ductal epithelium of the submucosal gland (d) and acini of the submucosal gland (a). (C&D)Representative immunofluorescence analyses of CB1 and EPCAM co-expression in two distinct human EAC samples. (E) Bar graph represents a comparative H-score semiquantitative intensity analysis of CB1 expression in human normal esophageal mucosa and EAC. The data were analyzed using the Mann-Whitney U test. ** p < 0.01.

Article Snippet: The blocking solution was replaced with the primary antibodies diluted in blocking solution using rabbit anti-CB1 (clone D5N5C, Cell Signaling Technologies, cat# 93815, Massachusetts, USA) and anti-epithelial cell adhesion molecule (EPCAM) (Origene, cat# UM500096, Maryland, USA), which was used to mark EAC.

Techniques: Immunofluorescence, Expressing, MANN-WHITNEY